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Broadband micro-vibration measurement for aerospace, vehicle, industrial inspection and earthquake prediction is urgently required, therefore a broadband and high precision IEPE accelerometer signal conditioner is designed in this paper. The Integral Electronics Piezoelectric(IEPE) accelerometer signal conditioner includes constant current source circuit, filter circuit, voltage follower circuit, single- ended to differential circuit and amplifier circuit. The IEPE accelerometer signal conditioner circuit board is printed. Then, the open-loop calibration experiment and closed-loop contrast experiment are carried out on the developed IEPE accelerometer signal conditioner circuit board. The open-loop calibration experimental results show that the phase difference of output signal is less than 2◦ , when input standard sinusoidal signal of 0.2Hz-1KHz to the developed IEPE accelerometer signal conditioner. The closed-loop contrast experimental results show that the root mean square(RMS) of error of the developed IEPE accelerometer signal conditioner and the same type signal conditioner of PCB company are about 0.0504mg. The developed IEPE accelerometer signal conditioner has broad application prospects in the field of broadband micro-vibration engineering.