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Bolted joints are widely applied for fastening the underframe equipment of the railway vehicles. The bolted joints directly or indirectly fixed on the vehicle body bear all kinds of vibration alternating loads. Firstly, the differences between Chinese and foreign relevant standards are compared and analyzed. Taking the bolted joints fastening the battery box hanging equipment of Electrical Multiple Unit (EMU) as an example, the overturning moment is introduced to be equivalent to the axial load of the bolt, and the strength calculation of the static load, fatigue load and shock load are carried out. Finally, the alternating load measured on the line is compared with the calculation results of fatigue strength to prove the reliability of the calculation method. Through the exploration of the strength check method for bolted joints, it provides references for the strength design of bolted joints of the EMU equipment.